The use of microbes to remove fluoride from water and soil is an extent of applied research and development. This is the first attempt have been made to examine physiochemical characteristics and also isolate fluoride resistant bacteria from ground waters in selected villages at Dindigul district, Tamil nadu, India. Based on high fluoride resistance (200 mM), three bacterial isolates were selected for further studies. The isolates authentically identified as genus Pseudomonas. Biochemical and 16S rRNA sequencing analysis of the isolates revealed that they are closely related (97%) to Pseudomonas sp. (98%) to Pseudomonas aeruginosa and (97%) to Pseudomonas sp. The 16S rRNA sequences were submitted in the NCBI under accession numbers MF481852, MF481853, MG751413. The fluoride resistant bacterial strains were resistant to antibiotics such as amoxicillin, ampicillin, chloramphenicol, kanamycin and streptomycin.