Clean water is vital for sustainable development, fostering socio-economic growth, ecological stability, and human survival. The study aimed to evaluate the quality of tube well water in specific residential areas of Khulna for drinking purposes, comparing it with the standards of World Health Organization (WHO) and Bangladesh (BD). Twenty tube well water sam-ples were collected from residential areas at depths of 700–1500 ft. The samples were tasted with about 20 parameters, including physical: color, taste, odor, total dissolved solids (TDS) and total suspended solids (TSS); chemical: acidity/alkalinity (pH), arsenic (As), electrical conductivity (EC), dissolved oxygen (DO), bicarbonate (HCO3–), calcium (Ca2+), magnesium (Mg2+), sodium (Na+), chloride (Cl–), iron (Fe), phosphate (PO43–), sulfate (SO42–), potassium (K+); and bacteriological parameters: total coliforms and E. coli bacteria. Most of the samples were found to have higher TDS (avg. 1380 mg/L), TSS (avg. 620 mg/L), Fe (avg. 20.2 mg/L), K+ (avg. 194.1 mg/L), and Na+ (avg. 439 mg/L) concentrations compared to the WHO and BD Standards. 90 to 95% of samples exhibited acceptable levels of EC, pH, DO, Mg2+, Ca2+, Cl–, and SO43–. The salinity levels in most of the samples were excessive to be used for drinking, and the levels were especially very high in samples S-01(Na+ 820 and Cl- 3195 mg/L) and S-04 (Na+ 660 and Cl- 2946 mg/L). The arsenic levels were found to be higher than acceptable limit in S-01 (0.086 mg/L) and S-04 (0.091 mg/L) as well. E. coli and other bacteria in a few samples (S-01, 04, 13, 16, 17, 19) were detected.